Single-molecule Technique Development

We are constantly developing new Single-molecule fluorescence (smFRET), single-molecule force spectroscopy (SMFS) and single-cell techniques for our research needs. We relies on these single-molecule techniques to help design and characterize molecular devices.

DNA-based Molecular Sensor / Machinery / Material

We design molecular sensors & molecular machines using DNA. DNAs are high programmable biomaterials with well defined folding behaviour. Simple (and complex!) molecular structures with novel functional properties can be designed, made and tested by single-molecule methods. Recently, we developed a fluorescent footprinting assay that allows us to see tiny forces at pico-newton level with single molecule resolution. We apply these assays to study important problems in cell adhesion. We are currently developing the next gen force sensors, molecular motor driving self-assembly and molecular devices using DNA.

Single-molecule & Single-cell Mechanobiology

We are constantly developing techinques and tools that enable us to understand the behaviour of cells from mutiple perspectives simultaneously - i.e. watching individual molecules do their jobs in a single cell, while monitoring how the cell respond to all these molecular events. We aim to correlate molecular to cellular behavior with spatial, temporal and functional resolutions. Much of this would be enabled by the development of molecular biosensors and new multimodal imaging techniques.

Exosome Biophysics and Bioengineering

We are currently developing new methods for tracking, isolating, and analyzing single exosomes for better disease diagnosis. This research is in close collaboration with colleagues in Engineering and the School of Medicine at UBC.